Abstract
A fully automated precolumn dericatization method for the determination of primary and secondary amino acids using reversed-phase chromatography is described. The derivatization reagent 9-fluorenyl methylchloroformate is used, together with 1-aminoadamantane for reaction with excess of reagent. The derivatization procedure is described in detail. Reproducibility data are presented and compared between the manual and the fully automated methods. Optimization strategies for separation, and differences in detection between fluorescence and UV methods are discussed. Analytical data for protein and peptide hydrolysates are compared with their sequenced data.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
