Full-Scale Demonstration of Nitrogen Removal from Mature Landfill Leachate Using a Two-Stage Partial Nitritation and Anammox Process

Abstract

The excessive discharge of nitrogen leads to water eutrophication. The partial nitritation and anammox (PN/A) process is a promising technology for biological nitrogen removal in wastewater treatment. However, applying it to mature landfill leachate (MLL) faces challenges, as the toxic substances (e.g., heavy metal) within MLL inhibit the activity of anammox bacteria. Therefore, most previous studies focused on diluted, pretreated, or chemically adjusted MLL. This study demonstrated at full scale that the two-stage PN/A process can treat raw MLL. Initially, the operational issue of sludge floatation resulted in rapid biomass loss with overflow discharging, which selectively suppresses nitrite-oxidizing bacteria (NOB), promoting the achievement of nitrite accumulation. After that, the NOB suppression was self-sustained by the high in situ free ammonia concentration, i.e., 26.2 ± 15.9 mg N/L. In the subsequent anammox tank, nitrogen removal primarily occurred via the anammox process, complemented by denitrification, achieving total nitrogen removal efficiency exceeding 72%. In addition, the nitrogen removal capacity of this system was significantly influenced by temperature with the nitrogen-loading rate above 0.4 kg N/m3/d at 38 °C and approximately 0.1 kg N/m3/d at 21 °C. The optimization of system operation, such as gradually increasing MLL content, remains necessary to enhance nitrogen removal capacity further.