Abstract
Iris lactea var. chinensis (I. lactea var. chinensis) is a perennial herb halophyte with salt and drought tolerance. In this study, full-length transcripts of I. lactea var. chinensis were sequenced using the PacBio RSII sequencing platform. Moreover, the transcriptome was investigated under NaCl or polyethylene glycol (PEG) stress. Approximately 30.89 G subreads were generated and 31,195 unigenes were obtained by clustering the same isoforms by the PacBio RSII platform. A total of 15,466 differentially expressed genes (DEGs) were obtained under the two stresses using the Illumina platform. Among them, 9266 and 8390 DEGs were obtained under high concentrations of NaCl and PEG, respectively. In total, 3897 DEGs with the same expression pattern under the two stresses were obtained. The transcriptome expression profiles of I. lactea var. chinensis under NaCl or PEG stress obtained in this study may provide a resource for the same and different response mechanisms against different types of abiotic stress. Furthermore, the stress-related genes found in this study can provide data for future molecular breeding.
Highlights
Plants are sessile organisms and are often affected by various abiotic stresses during their life cycle
Based on benchmarking universal single-copy ortholog (BUSCO) analysis, approximately 307 (72%) of the 429 expected embryophyte genes were identified as complete (Supplementary Materials Table S1)
The results showed that a total of 22 differentially expressed genes (DEGs) were mapped to the abscisic acid (ABA) signaling pathway, of which six DEGs were mapped to the ABA receptor PYRs/PYL, and all of them were in a downward trend; five DEGs were mapped to the threonine/serine receptor kinase
Summary
Plants are sessile organisms and are often affected by various abiotic stresses during their life cycle. These abiotic stresses include drought, salt damage, and toxic metals in soil, among which salt damage and drought stress are important factors limiting plant growth and productivity [1]. Intensive agricultural irrigation will lead to changes in soil water balance in these areas and aggravate soil salinization [2]. It is critical to increase plant resistance to drought and salt for agricultural production and the sustainable use of the environment. The water and fertilizer consumption of stress-resistant plants will be greatly reduced, reducing the cost and environmental burden
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