Abstract
Plant cell totipotency is one of the 25 major topics in current scientific research, and somatic embryos are good experimental material for studying cell totipotency. Polar auxin transport plays an important regulatory role in somatic embryogenesis (SE). However, little is known about the auxin transport genes and their regulatory mechanisms in Lilium SE. In this study, we applied single-molecule real-time (SMRT) sequencing to Lilium pumilum DC. Fisch. for the first time and obtained a total of 119,649 transcripts, of which 14 encoded auxin transport genes. Correlation analyses between somatic embryo induction and gene expression under different treatments revealed that auxin transport genes, especially ATP-binding cassette (ABC) transporter B family member 21 (ABCB21) and PIN-FORMED (PIN) LIKES 7 (PILS7), may be key players in SE, and the necessary duration of picloram (PIC) treatment to induce SE is as short as 3 days. Our research provides valuable genetic information on Lilium pumilum, elucidating the candidate auxin transport genes involved in SE and their influencing factors. This study lays a foundation for elucidating the regulatory mechanism of auxin transport in SE.
Highlights
Like animals, plants have complex mechanisms that regulate cell division, development and growth [1]
The members of the ATP-BINDING CASSETTE B-TYPE (ABCB) subfamily, PIN/PILS family and AUXIN RESISTANT/LIKE AUXIN RESISTANT (AUX/LAX) family, which participate in polar auxin transport were identified and screened, and 14 genes involved in polar auxin transport were cloned for the first time in Lilium pumilum
Owing to its long reads, high sensitivity, lack of GC bias and ability to directly detect modified bases, single-molecule real-time (SMRT) sequencing has solved several major problems associated with RNA-seq
Summary
Plants have complex mechanisms that regulate cell division, development and growth [1]. An efficient and synchronized somatic embryo regeneration system provides an important molecular basis for studying the transition from somatic to embryonic cells [5] During this transition, dedifferentiation is needed to stimulate cell division and physiological, metabolic and gene expression [5,6]. The members of the ABCB subfamily, PIN/PILS family and AUX/LAX family, which participate in polar auxin transport were identified and screened, and 14 genes involved in polar auxin transport were cloned for the first time in Lilium pumilum. This article provides a relatively complete and accurate reference transcriptome for SE or even other biological processes of Lilium pumilum and preliminarily confirms that water stress may be one of the reasons why somatic cells regain totipotency, while polar auxin transport promotes the development of totipotent cells into somatic embryos
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