Abstract
The emergence and rapid spread of unusual DS-1-like intergenogroup reassortant rotaviruses having G1/3/8 genotypes have been recently reported from major parts of the world (Africa, Asia, Australia, Europe, and the Americas). During rotavirus surveillance in Thailand, three novel intergenogroup reassortant strains possessing the G9P[8] genotype (DBM2017-016, DBM2017-203, and DBM2018-291) were identified in three stool specimens from diarrheic children. In the present study, we determined and analyzed the full genomes of these three strains. On full-genomic analysis, all three strains were found to share a unique genotype constellation comprising both genogroup 1 and 2 genes: G9-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis demonstrated that each of the 11 genes of the three strains was closely related to that of emerging DS-1-like intergenogroup reassortant, human, and/or locally circulating human strains. Thus, the three strains were suggested to be multiple reassortants that had acquired the G9-VP7 genes from co-circulating Wa-like G9P[8] rotaviruses in the genetic background of DS-1-like intergenogroup reassortant (likely equine-like G3P[8]) strains. To our knowledge, this is the first description of emerging DS-1-like intergenogroup reassortant strains having the G9P[8] genotype. Our observations will add to the growing insights into the dynamic evolution of emerging DS-1-like intergenogroup reassortant rotaviruses through reassortment.
Highlights
Group A rotavirus (RVA) within the Reoviridae family, is the primary pathogen that causes severe gastroenteritis in young children and animals worldwide
Since 2008, a full genome-based genotyping system based on assignment of all the 11 gene segments has been introduced, where Gx-P[x]Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx designates the genotypes of the VP7-VP4-VP6-VP1-VP2VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes [5, 6]
The nucleotide sequences of the full genomes of five locally circulating human RVAs (HuRVAs) strains (three G2P[4] strains with a short electropherotype (DBM2017-003, DBM2017-015, and DBM2018-105) and two G9P[8] strains with a long electropherotype (DBM2017-014 and DBM2018-111)) detected in stool specimens from diarrheic children admitted to Bhumibol Adulyadej Hospital were determined as well, as references
Summary
Group A rotavirus (RVA) within the Reoviridae family, is the primary pathogen that causes severe gastroenteritis in young children and animals worldwide. The segmented nature of the genome facilitates reassortment between/among RVA strains, and the reassortment plays one of the major roles in the dynamic evolution of RVAs [4]. The emergence and rapid spread of uncommon DS-1-like intergenogroup reassortant strains, i.e., DS-1-like G1P[8] strains and their derivatives having genotype constellations G1/3/ 8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2, have been recently reported from major parts of the world (Africa, Asia, Australia, Europe, and the Americas) [6, 10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
