Full composition granules of Huanglian (Rhizoma Coptidis) decrease the serum monocyte chemotactic protein-1 and connective tissue growth factor levels and inhibit kidney nuclear factor-κB expression in rats with high-fat diet-induced diabete.

Abstract

To investigate the efficacy of the full composition granules of Huanglian (Rhizoma Coptidis) (FGC) on the serum monocyte chemotactic protein-1 (MCP-1) and connective tissue growth factor (CTGF) levels and kidney nuclear factor-κB (NF-κB) expression in rats with high-fat diet-induced diabetes. Diabetes was induced in rats by feeding a high-fat chow combined with intravenous streptozotocin injection. Forty diabetic Sprague- Dawley rats were randomly assigned to a normal group (NG), model group (MG), irbesartan group (IG), and low-, middle-, and high-dosage FGC groups (LFGC, MFGC, HFGC), with eight rats per group. The IG rats received 31.25 mg·kg－1·d－1 irbesartan tablets, whereas those in the LFGC, MFGC, and HFGC were administered 52, 312.5, and 625 mg·kg－1·d－1 FGC, respectively. After 12 weeks, bodyweight (BW), left kidney weight (KW), hemoglobin A1c (HbA1c), serum creatinine (Scre), blood urea nitrogen (BUN), and serum MCP-1 and CTGF levels were determined, pathological changes of the kidney were recorded, and kidney NF-κB p65 (A) expression was measured. The 24-h urine albumin and levels of HbA1c, Scre, BUN, and serum MCP-1 and CTGF were significantly increased in in the MG compared with the NG, as was the kidney NF-κB (p65) expression (P < 0.05). Furthermore, clear pathological changes in kidney fibrosis were observed in the MG rats. Following irbesartan and FGC administration, the 24-h urine albumin and the levels of HbA1c, Scre, and serum MCP-1 and CTGF were significantly decreased in FCG groups compared with those in the MG, which is in agreement with the change in the kidney NF-κB (p65) expression, whereas the similarly significant decrease only exist in 24-h urine albumin and the levels of serum CTGF after irbesartan administration. Hematoxylin-eosin (HE) staining results indicated that the fibrosis observed in the MG samples was alleviated through FGC treatment. FGC may alleviate potential kidney injury by decreasing the serum MCP-1 and CTGF levels and inhibiting NF-kB expression in diabetic nephropathy in rats with high-fat diet-induced diabetes.