Fucoxanthin enhances 5-FU chemotherapeutic efficacy in colorectal cancer cells by affecting MMP-9 invasive proteins

Abstract

Background : Drug resistance is a major inconvenient lowering the traditional chemotherapeutic efficacy and highly undesirable therapeutic problem which crucially editing faster in colorectal cancer. Fucoxanthin is a naturally orange-carotenoid, predominantly found in edible brown algae and justify considered as nutritional ingredient possess with powerfully new strategy to enhance concurrent drug chemotherapy. Obviously studied exhibited that fucoxanthin is very good well documented as potential anti-cancer activity, remarkable numerous biological activities. Accordingly, it has prominently gain research to enhancing the understand in molecular mechanism details associating cancer therapy. Objectives : This study was undertaken to assess the anti-cancer activity and explored the molecular mechanism of fucoxanthin on the inhibition of cell proliferation and cell invasion. Methods : SW-620 cells were cultivated with fucoxanthin with for 24, 48, 72, 96 hr and co-treatment with 5-FU to evaluate synergistic potential. The cell viability of cancerous cells were determined by MTT colorimetric assay. The inhibitory effect of cell invasion and adhesion was measured in the presence of fucoxanthin with 5-FU in various concentrations. To determine MMP-9 gene and protein expression after treated cells by RT-PCR and ELISA assay Results : The results illustrated that fucoxanthin profoundly inhibited cell proliferation of SW-620 cells accompanied by growth arrest and diminished invasive ability, which mediated at one least part by the down-regulation of MMP-9 mRNA and protein expression. Especially, fucoxanthin extremely attenuated the anti-proliferative effect of established 5-FU by modulating the habitually hallmark of cancerous cells. Conclusions : This results are raising up the capacity of fucoxanthin to eradicate cancer cells and the possibility that fucoxanthin could become a promising marine natural active constituents from seaweeds. Critical outcome of data in our studies will be serving as preliminary results for further studies marine drug in experimental model and well-controlled clinical trials.