Fucoxanthin-Containing Cream Prevents Epidermal Hyperplasia and UVB-Induced Skin Erythema in Mice.

Azahara Rodríguez-Luna,Elena Talero,Virginia Motilva,María Cózar,María González-Rodríguez,Javier Ávila-Román,Antonio Rabasco

doi:10.3390/md16100378

Azahara Rodríguez-Luna, Elena Talero + Show 5 more

Open Access

https://doi.org/10.3390/md16100378

Copy DOI

Journal: Marine drugs	Publication Date: Oct 10, 2018
Citations: 61	License type: CC BY 4.0

Affiliation: Universidad de Sevilla

Abstract

Microalgae represent a source of bio-active compounds such as carotenoids with potent anti-inflammatory and antioxidant properties. We aimed to investigate the effects of fucoxanthin (FX) in both in vitro and in vivo skin models. Firstly, its anti-inflammatory activity was evaluated in LPS-stimulated THP-1 macrophages and TNF-α-stimulated HaCaT keratinocytes, and its antioxidant activity in UVB-irradiated HaCaT cells. Next, in vitro and ex vivo permeation studies were developed to determine the most suitable formulation for in vivo FX topical application. Then, we evaluated the effects of a FX-containing cream on TPA-induced epidermal hyperplasia in mice, as well as on UVB-induced acute erythema in hairless mice. Our results confirmed the in vitro reduction of TNF-α, IL-6, ROS and LDH production. Since the permeation results showed that cream was the most favourable vehicle, FX-cream was elaborated. This formulation effectively ameliorated TPA-induced hyperplasia, by reducing skin edema, epidermal thickness, MPO activity and COX-2 expression. Moreover, FX-cream reduced UVB-induced erythema through down-regulation of COX-2 and iNOS as well as up-regulation of HO-1 protein via Nrf-2 pathway. In conclusion, FX, administered in a topical formulation, could be a novel natural adjuvant for preventing exacerbations associated with skin inflammatory pathologies as well as protecting skin against UV radiation.

Highlights

IntroductionThe normal structure and functionality could be altered by external factors such as toxic compounds or ultraviolet (UV) radiation, or by internal factors including genetic predisposition, immune and hormone disorders, or stress
Skin is the organ that acts as main defence against external environment factors, protecting the organism from harmful substances, mechanical damage, pathological invasion and radiations.the normal structure and functionality could be altered by external factors such as toxic compounds or ultraviolet (UV) radiation, or by internal factors including genetic predisposition, immune and hormone disorders, or stress
Once a topical formulation was selected, we aimed to study its effect on the TPA-induced hyperplasia model in mice, which mimics psoriatic parameters in dorsal murine skin, and on the UVB-induced erythema model in hairless mice, which reproduces the consequences expected in humans receiving acute UVB radiation

Summary

Introduction

The normal structure and functionality could be altered by external factors such as toxic compounds or ultraviolet (UV) radiation, or by internal factors including genetic predisposition, immune and hormone disorders, or stress. The result of these skin perturbations could trigger an inflammatory process, an oxidative stress status, an unbalanced epidermal homeostasis, or a limited immune response, among others [1]. UV skin exposure to treat these inflammatory conditions is recommended due to its beneficial effects In this line, UV radiation achieves long remission periods in psoriasis through activation of immunosuppressive pathways and keratinocyte apoptosis [3].

Objectives

Methods

Results

Discussion

Conclusion