Fucoxanthin and Rosmarinic Acid Combination Has Anti-Inflammatory Effects through Regulation of NLRP3 Inflammasome in UVB-Exposed HaCaT Keratinocytes.

Azahara Rodríguez-Luna,Elena Talero,Javier Ávila-Román,Helena Oliveira,Virginia Motilva

doi:10.3390/md17080451

Azahara Rodríguez-Luna, Elena Talero + Show 3 more

Open Access

https://doi.org/10.3390/md17080451

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Journal: Marine Drugs	Publication Date: Aug 1, 2019
Citations: 66	License type: CC BY 4.0

Affiliation: Universidad de Sevilla, University of Aveiro

Abstract

Excessive exposure to ultraviolet (UV) radiation is the main risk factor to develop skin pathologies or cancer because it encourages oxidative condition and skin inflammation. In this sense, strategies for its prevention are currently being evaluated. Natural products such as carotenoids or polyphenols, which are abundant in the marine environment, have been used in the prevention of oxidative stress due to their demonstrated antioxidant activities. Nevertheless, the anti-inflammatory activity and its implication in photo-prevention have not been extensively studied. Thus, we aimed to evaluate the combination of fucoxanthin (FX) and rosmarinic acid (RA) on cell viability, apoptosis induction, inflammasome regulation, and anti-oxidative response activation in UVB-irradiated HaCaT keratinocytes. We demonstrated for the first time that the combination of FX and RA (5 µM RA plus 5 μM FX, designated as M2) improved antioxidant and anti-inflammatory profiles in comparison to compounds assayed individually, by reducing UVB-induced apoptosis and the consequent ROS production. Furthermore, the M2 combination modulated the inflammatory response through down-regulation of inflammasome components such as NLRP3, ASC, and Caspase-1, and the interleukin (IL)-1β production. In addition, Nrf2 and HO-1 antioxidant genes expression increased in UVB-exposed HaCaT cells pre-treated with M2. These results suggest that this combination of natural products exerts photo-protective effects by down-regulating NRLP3-inflammasome and increasing Nrf2 signalling pathway.

Highlights

Skin is considered as the outmost protective barrier in the body, protecting from detrimental substances, mechanical damage, pathological invasion and radiation that could cause perturbations to the skin structure
These results suggest that this combination of natural products exerts photo-protective effects by down-regulating NRLP3-inflammasome and increasing
To further elucidate the photo-protective mechanism of the combination of rosmarinic acid (RA) and FX, we evaluated the capacity to activate antioxidant pathways as Nrf2, which promotes the transcription of evaluated the capacity to activate antioxidant pathways as Nrf2, which promotes the transcription of antioxidant genes and detoxification of enzymes such as heme-oxygenase 1 (HO-1) to protect against antioxidant genes and detoxification of enzymes such as heme-oxygenase 1 (HO-1) to protect against an oxidative damage as UVB exposure [28]

Summary

Introduction

Skin is considered as the outmost protective barrier in the body, protecting from detrimental substances, mechanical damage, pathological invasion and radiation that could cause perturbations to the skin structure. In this sense, skin is a well-known essential piece of the immune system. Several factors can contribute to the initiation and development of cutaneous alterations. In this line, the excessive exposure to UV radiation remains the main risk factor for the skin cancer [1]. UVB causes dermal changes, affects epidermal function and is the main factor

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