Fucoidan-loaded hydrogels facilitates wound healing using photodynamic therapy by in vitro and in vivo evaluation

Abstract

The present study aimed at fabrication and evaluation of carboxymethyl cellulose nanofibril-loaded fucoidan/alginate-based gellan gum hydrogel for wound healing with photodynamic approaches. The healing effect of multifunctional hydrogel was characterized and evaluated with in vitro and in vivo methods. Skin tissues were used to perform histological and immunohistochemical analysis to evaluate fibroblasts and collagen content. Protein levels were detected using Western blots. The results proved that, upon laser irradiation, hydrogel led to a significant increase in cell death and cell viability because of non toxic and functional groups in spherical nanosize structure. The hydrogel can destroy cancer cells by singlet oxygen with increased reactive oxygen species levels. Faster wound healing with increase in fibroblast and collagen fibers of high protein expression was observed. Thus, these findings suggest that hydrogel is a potent therapeutic agent that can be used to promote efficient and rapid wound healing for treatment of skin wounds.