Fucoidan isolated from invasive Sargassum horneri inhibit LPS-induced inflammation via blocking NF-κB and MAPK pathways

Abstract

The brown seaweed Sargassum horneri, recently invaded the shorelines or Jeju, South Korea. Within the framework of a sustainable strategy to control S. horneri, authors attempted to evaluate extraction yield, spectroscopic characterization, and anti-inflammatory properties of fucoidan, a commercially valuable sulfated polysaccharide, isolated from S. horneri. Previously, a number of studies reported that sulfated polysaccharides (SP) present in S. horneri is a potential candidate to develop as a functional ingredient. However, anti-inflammatory mechanisms and or SP responsible for those effects of S. horneri yet to be exposed. In, the present study, the authors attempted to isolate bioactive SP using Celluclast enzyme-assisted extraction followed by FPLC system assisted purification. The purified polysaccharide (SHCF2) inhibited the LPS-stimulated NO production in RAW 264.7 cells (IC50 = 40 μg/mL) via the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signal pathways. Specifically, SHCF2 down-regulated the heart-beating rate, cell death, ROS, and NO levels in LPS-exposed zebrafish embryo. In conclusion, our results expressed that this invasive brown seaweed is a potential candidate to isolate fucoidans.