Fucoidan from Undaria pinnatifida Ameliorates Epidermal Barrier Disruption via Keratinocyte Differentiation and CaSR Level Regulation.

Yu Chen,Nuo Xu,Junmei Qi,Meiling Tai,Xuenan Li,Shuang Gao,Zhenlin Hu,Xiaoshuang Gan,Wengang Zhao,Biao Che

doi:10.3390/md17120660

Abstract

The epidermal barrier acts as a line of defense against external agents as well as helps to maintain body homeostasis. The calcium concentration gradient across the epidermal barrier is closely related to the proliferation and differentiation of keratinocytes (KCs), and the regulation of these two processes is the key to the repair of epidermal barrier disruption. In the present study, we found that fucoidan from Undaria pinnatifida (UPF) could promote the repair of epidermal barrier disruption in mice. The mechanistic study demonstrated that UPF could promote HaCaT cell differentiation under low calcium condition by up-regulating the expression of calcium-sensing receptor (CaSR), which could then lead to the activation of the Catenin/PLCγ1 pathway. Further, UPF could increase the expression of CaSR through activate the ERK and p38 pathway. These findings reveal the molecular mechanism of UPF in the repair of the epidermal barrier and provide a basis for the development of UPF into an agent for the repair of epidermal barrier repair.

Highlights

The outermost layer or epidermis of the skin serves to perform the majority of the functions of the skin such as the first line of defense against the environment
The distribution of the monosaccharide molar ratio of Undaria pinnatifida (UPF) is presented as mannose: rhamnose: galactose: fucose = 11.7:4.14:12.7:7.49 (Figure 1B)
The results revealed that the could alleviate the symptom of epidermal hyperplasia during the recovery process of the barrier epidermal thickness of 0.5% and 5% UPF treated mice was much less than that of model mice, Mar

Summary

Introduction

The outermost layer or epidermis of the skin serves to perform the majority of the functions of the skin such as the first line of defense against the environment. This epidermis functions in defense against toxic and pathogenic agent entry and suppresses the loss of water, ions, and metabolites. 90%–95% of epidermal cells are keratinocytes making up the chief type of cell in the epidermal barrier. These cells undergo division in the basal layers to later differentiate across the epidermis to become SC cells that are enucleated and flattened which will desquamate eventually. The process of differentiation in the epidermis involves the development of numerous layers of keratinocytes from the stratum basale to the stratum spinosum and the stratum

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