Abstract
The vibrational spectra of eight genomic DNAs from leaf tissues (sword fern (Nephrolepis exaltataL.), chrysanthemum (Dendranthema grandifloraRamat.), redwood (Sequoia sempervirensD. Don. Endl.), orchids (Cymbidium × hybrida), common sundew (Drosera rotundifoliaL.), potato (Solanum tuberosumL.) and scopolia (Scopolia carniolicaJacq.)) have been analyzed using FT-Raman spectroscopy, in the wavenumber range 500–1800 cm–1.FT-Raman signatures, spectroscopic assignments and structural interpretations for these plant genomic DNAs are reported. Spectral differences among two genomic DNAs, independently extracted from chrysanthemum leaves, are to be observed between 1000–1200 cm–1. Besides, similarities in the FT-Raman spectra of genomic DNAs from potato and scopolia leaves, respectively, have been found. This might be explained by their belonging to the same family (Solanaceae). Other spectral differences among genomic plant DNAs have also been observed.These findings demonstrate that Raman spectroscopy may be exploited to distinguish different plant genomic DNAs.The present study provides a basis for future use of Raman spectroscopy to analyze specific plant DNA–ligand interactions or DNA structural changes induced by plants' stress conditions associated with their natural environment.
Highlights
Plants are subjected to a variety of stress conditions associated with their natural environment
These findings demonstrate that Raman spectroscopy may be exploited to distinguish different plant genomic DNAs
The present findings demonstrate that Raman spectroscopy may be exploited to distinguish different plant genomic DNAs
Summary
Plants are subjected to a variety of stress conditions associated with their natural environment. Structural features of nucleic acids in biological assemblies are of particular importance, because they can reflect these types of changes. Raman spectroscopy offers certain advantages for the investigation of structural, dynamical, thermodynamic and kinetic properties of DNA [1]. This approach can provide definitive information about covalent bonding configurations and is potentially informative of electrostatic, hydrophobic, and hydrogen-bonding interactions involving specific nucleotide subgroups [2]. The utility of the method to distinguish binding of divalent cations to DNA, to establish the low pH-induced DNA structural changes [3,4,5,6], to monitor thermal denaturation of DNA and DNA/metal-ion complexes, and
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