FTIR investigation of spray-dried milk protein concentrate powders

Abstract

Fourier transform infrared (FTIR) spectroscopy was used to examine the conformation of proteins in spray-dried milk protein concentrate (MPC) powders and to determine if the spectral changes could be related to nitrogen solubility of these powders. MPC samples (83–92% protein, dry basis) were prepared using a range of processing conditions and stored for 4 weeks at 21 °C. FTIR spectra were collected in the mid infrared (MIR) region between 4000 and 600 cm −1. FTIR data was pre-processed to remove physical effects causing discrimination between samples using firstly second derivatives and normalization and secondly the extended multiplicative scatter correction (EMSC) technique. The FTIR spectral changes were subsequently assessed using second derivative spectroscopy and principal components analysis (PCA) in the amide I and II regions (1700–1400 cm −1) and the fingerprint region (1800–700 cm −1). PCA analysis showed that the different powder preparations could be separated on scores plots but the separation was not related to nitrogen solubility per se. However, changes in nitrogen solubility of individual MPC powders during storage could be correlated to changes in FTIR spectra. PCA analysis of FTIR spectra could generally discriminate between MPC powders that had lost significant nitrogen solubility (9–20%) and those in which nitrogen solubility was preserved on storage. There were changes in intensity and/or position of bands at 1630 cm −1 when the solubility of a stored sample decreased substantially. The results of this work also show that EMSC data pre-processing for these samples gives comparable results when compared with more complicated data pre-processing for the removal of physical effects.