FT-IR Characterization of Pollen Biochemistry, Viability, and Germination Capacity inSaintpauliaH. Wendl. Genotypes

Abstract

FT-IR characterization of pollen biochemistry was analyzed to detect possible connection with the viability (by staining with potassium iodide, 25%) and the germination capacity (on solid nutrient medium), in 15Saintpauliagenotypes. Vibrational spectroscopy indicates that the pollen ofS. ionanthagenotype “Red Velvet” is rich in proteins, lipids, triglycerides, and esters and has a viability of 88.4% and a low germination capacity (27.16%). ForS. ionantha“Jolly Red” and “Lucky Ladybug” genotypes, pollen showed high viability (88.81–91.49%) and low germination capacity (23.02–9.17%), even though the pollen is rich in carbohydrates.S. ionantha“Aloha Orchid” genotype has the highest percentage of viability (94.32%) and germination capacity (45.73%) and a rich content of carbohydrates and polygalacturonic acids. InS. rupicolaandS. ionanthagenotypes, the rich content of polygalacturonic acids, lipids, and carbohydrates favourably influenced the germination capacity. Spectroscopic result indicates, through different absorbance band intensity, a possible link between biochemical composition, viability, and germination capacity ofSaintpauliapollen. To determine exactly the relation between biochemistry and biological processes, it is necessary to initiate quantitative researches.