Abstract
FT-IR/ATR analytical technique is one of the most applicable techniques worldwide. It is closely associated with easy-to-use equipment, rapid analysis, and reliable results. This study reports the simultaneous qualitative and quantitative analysis of two active pharmaceutical ingredients (APIs), of a piperacillin and tazobactam formulation using a film formation method. This method requires film formation on the ATR crystal, resulting from solvent evaporation of a small amount of liquid sample. Good contact between the film and the crystal led to the identification of both APIs, although tazobactam was of low content in the formulation mixture. The quantification of the APIs in the commercial mixture was also achieved, using a single calibration line with a correlation coefficient equal to 0.999, not only after film formation but also in the initial dry formulation before reconstitution. The present spectroscopic technique combined with the proposed relatively simple sample treatment outweighs chromatographic protocols already applied, which require specialized staff and are costly, time-consuming, and not environmentally friendly. Taking all the above into consideration, it turns out that such an approach has the potential to be used for off-line quality control procedures in manufacture or, in terms of portable equipment and automated software, anywhere for on-site analysis, even in a hospital workflow.
Highlights
Fourier Transform Infrared Spectroscopy (FT-IR) is a well-established analytical technique.Mid-IR region (4000–600 cm−1 ) offers the ‘’fingerprint” of the analyte, as it is rich in information about the structure of the functional groups of the sample tested [1]
This paper reports on the employment of the film-formation, preparation technique for the qualitative and quantitative analysis of a piperacillin (PIP)
FT-IR/ATR spectra of the powder of both the pure active substances were obtained (Figure 1) and some of the most significant peaks of them were attributed to the vibration modes of their molecules (Table 1) [1]
Summary
Mid-IR region (4000–600 cm−1 ) offers the ‘’fingerprint” of the analyte, as it is rich in information about the structure of the functional groups of the sample tested [1]. Apart from that, it can be applied for quantitative analysis as the energy absorbed at a particular wavelength is in proportion with the number of bonds absorbing the associated quanta of energy [2]. In a typical ATR experiment, the incident infrared radiation interacts with the analyte molecules on the crystal surface via an evanescent wave created by total reflectance in the crystal. The attenuation of the evanescent field by the absorption in the sample is detected.
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