FSH Receptor and Ki-67 Protein Expressions of Endometrial Macaque in Mid-Luteal Phase after Exposure to Various Doses of Recombinant FSH and Fixed-Dose of GnRH Agonist

Nurhuda Sahar,Supri Irianti Handayani,Rosalina Thuffi,Raden Muharram,Rijal Subahar,Ponco Birowo,Andhea Debby Pradhita,Fatmawaty Yazid,Vivitri Dewi Prasasty,Wa Ode Zulhulaifah

doi:10.3844/ojbsci.2021.260.266

Abstract

FSH Receptor and Ki-67 Protein Expressions of Endometrial Macaque in Mid-Luteal Phase after Exposure to Various Doses of Recombinant FSH and Fixed-Dose of GnRH Agonist

Highlights

Implantation failure is still one of the main factors that limit the success of in vitro Fertilization (IVF) (Simon and Laufer, 2012)
It can be concluded that macaque endometrial cells were receptive to the given dosages of recombinant Follicle-Stimulating Hormone (rFSH)
The Procedure of Controlled Ovarium Hyperstimulation (COH). These macaques were divided into five groups according to their Controlled Ovarian Hyperstimulation (COH) protocols, including a group of GonadotropinReleasing Hormone (GnRH) agonist administration with a dose of 160 μg/day; groups of rFSH with dosages of 30, 50, 70 and a control group

Summary

Introduction

Implantation failure is still one of the main factors that limit the success of in vitro Fertilization (IVF) (Simon and Laufer, 2012). Two main factors play a role in the success of IVF, including oocyte quality and endometrial receptivity when receiving blastocyst implantation in the endometrium (Simon et al, 2000; Sakiner et al, 2018). The use of gonadotropins in COH procedures can negatively affect the endometrial maturation of the implantation period (Kolibianakis et al, 2002; Sahar et al, 2019) It happens because gonadotropins increase progesterone and estradiol hormones in the blood serum (Lai et al, 2009). Our previous study revealed that after a fixed-dose administration of GnRH agonist and various rFSH doses, 60% of the implantation window was closed (Sahar et al, 2019) It is currently unknown the impact of high-dose of rFSH administration on Follicle-Stimulating Hormone Receptor (FSHR) and Ki-67 protein expression on endometrial cell proliferation, which is one of the important determinants of implantation window. The purpose of this study was to analyze the development of Macaca nemestrina endometrium after COH by assessing the expression of cell proliferation markers and their correlation with the expression of FSHR and Ki-67 protein

Materials and Methods

Results

Discussion

Conclusion