Abstract
Molecular distribution profiles for follicle-stimulating hormone (FSH), luteinizirig hormone (LH), and thyroid-stimulating hormone (TSH) in anterior pituitary extracts from intact and gonadectomized rats of both sexes, androgen-treated orchidectomized rats, and estrogen-treated ovariectomized rats were obtained by exclusion chromatography. Hormonal activities of the extracts and of the eluted fractions were determined by radioimmunoassay. Two internal “markers”, serum albumin (SA) and ovalbumin (OA), were added to each aliquot of pituitary extract before it was applied to the column. The locations of the FSH, LH and TSH peaks were plotted in relation to those of these two standard proteins. The elution position of TSH was relatively constant; it invariably peaked just ahead of the OA marker, behind FSH and LH. Generally, LH also varied little in location, although the LH from female rats did tend to be retarded slightly. By contrast, there were distinct differences among the FSH peaks. FSH from males and ...
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