FSCN1 is an effective marker of poor prognosis and a potential therapeutic target in human tongue squamous cell carcinoma

Yue Chen,Hao Li,Chun-Yang Wang,An-Kui Yang,Yan-Feng Chen,Zhi-Yong Li,Wei-Ye Deng,Tian Tian,Rong Deng

doi:10.1038/s41419-019-1574-5

Abstract

To estimate the value of FSCN1 in evaluating the prognosis and guiding the targeted therapy for patients with tongue squamous cell carcinoma (TSCC). Using the Oncomine database, we found some genes especially FSCN1 differentially expressed between TSCC samples and tongue normal samples. So we compared FSCN1 expression between TSCC and normal cell lines and knocked down FSCN1 in TSCC cells to observe its influence on the viability and trans-migration in vitro and tumor growth in vivo. Then we measured FSCN1 expression in human cancer tissues and adjacent non-carcinoma tissues (ANT) and explored the relationship between FSCN1 expression and clinical pathological factors and prognosis in TSCC patients. We found that FSCN1 is expressed higher in TSCC cells than in normal cells. Knockdown of FSCN1 reduced TSCC cell viability and trans-migration in vitro and impaired tumor growth in vivo. FSCN1 also expressed higher in human TSCC than in ANT. In addition, FSCN1 expression was related to N classification, clinical stage and relapse. TSCC patients with over-expression of FSCN1 had worse prognosis. In conclusion, over-expression of FSCN1 indicates worse prognosis for patients with TSCC and FSCN1 may be a potential prognostic biomarker and therapeutic target in TSCC.

Highlights

Tongue squamous cell carcinoma (TSCC) is one of the most common oral squamous cell carcinomas worldwide
Expression of Fascin actin-bundling protein 1 (FSCN1) in TSCC from Oncomine database Using the Oncomine database, we found a study by Estilo et al indicated that the FSCN1 gene expressed 7.42times higher in TSCC samples (31 samples) than in tongue normal samples (26 samples) (Fig. 1a)
These studies all agreed that FSCN1 expression was higher in TSCC samples than in tongue normal samples (p < 0.05)

Summary

Introduction

Tongue squamous cell carcinoma (TSCC) is one of the most common oral squamous cell carcinomas worldwide. The development of TSCC is a complicated and multistep process that includes a loss of cell cycle control, which may be due to. From the Oncomine database, we found that the FSCN1 gene expressed much higher in TSCC samples than in tongue normal samples. Aberrant expression of Fascin actin-bundling protein 1 (FSCN1) encodes a member of the Fascin family of actin-binding proteins. Fascin organizes F-actin into parallel bundles and is required for the formation of actin-based cellular protrusions[4]. Recent studies have shown that Fascin plays a critical role in cell migration, motility, adhesion, and cellular interactions[5,6]. The up-regulation of Fascin protein expression has been

Methods

Results

Conclusion