Fruit ripening and chitosan coating impacts on the expression profile of Banana Lectin (BanLec) genes

Abstract

Banana Lectin (BanLec) was found in banana, is capable to identify and attach to carbohydrates without modified them. While the process for the synthesis of lectins remains unidentified, it is understood that certain inducing factors can trigger their expression. This research assessed the gene expression levels of lectins in Cavendish bananas throughout different stages of ripening by contrasting bananas with peels coated in 1.25 % chitosan solution to their uncoated counterparts. This experiment was performed using a transcriptomic approach and conducted in silico. The in silico analysis revealed the induction of 14 distinct lectin genes expression subsequent to the ripening stages in uncoated Cavendish bananas. Along with the ripening process, it was observed that lectins potentially contribute to protein phosphorylation and augment the carbohydrate-binding activity. Conversely, the application of a 1.25 % chitosan coating led to the heightened expression of 17 specific lectin genes. Post-coating, it is hypothesized that modifications to the primary cell wall were effected through the cleavage and recombination of xyloglucan molecules. The qPCR results substantiated the induction of BanLec genes, Ma09_g10350 and Ma11_g22300, by the application of chitosan coating. Findings from this transcriptomic investigation propose that the upregulation of lectin genes induced after chitosan treatment may be implicated in plant defense mechanisms. This study reveals that natural ripening and a 1.25 % chitosan coating influenced lectin gene expression in Cavendish bananas differently. Both ripening and chitosan treatments appear to be viable strategies for enhancing banana lectin expression, which has potential health applications.