Abstract
Conventional lung preparatory methods for electron microscopy utilizing chemical fixation, dehydration, embedding and/or drying have been critical for understanding alveolar structure. However, these procedures cause the removal of all fluids, alteration of dimensions, and distortion of sp. tial relationships and therefore limit the information that can be obtained. Freeze fracture replica techniques, on the other hand, have provided information on frozen hydrated alveolar structures, but replica procedures require removal of all tissue and cannot preserve the convoluted topography of entire inflated alveoli. Described here is an alternative lung preservation method for use in freeze-fracture, low temperature scanning electron microscopy (SEM) which preserves alveoli in the frozen-hydrated state and permits direct observation of entire inflated alveoli.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
