Front Cover: Simple and rapid analysis of Linagliptin in dried blood spot using an ionic liquid based vortex‐assisted dispersive liquid–liquid microextraction coupled with liquid chromatography–electrospray ionization–tandem mass spectrometry: Application to pharmacokinetic studies

Abstract

DOI: 10.1002/sscp.202100008 The cover picture shows a simple, rapid and convenient ionic liquid based vortex-assisted dispersive liquid-liquid microextraction method for the determination of Linagliptin. The extraction sample was assayed using liquid chromatography tandem-mass spectrometry using electrospray ionization. The effects of significant factors in extracting and disperser solvent, and salt contents were investigated. MS/MS detection was performed to monitor precursor → product ion transitions m/z 473.3 → 364.2 for Linagliptin, m/z 408.2 → 235.1 for internal standard. The assay was linear from 6–1500 ng/mL and the mean recoveries are more than 90%. The peak dried blood spot concentration (Cmax) after 1.5 h was determined to be 1391.2 ng/mL for Linagliptin.