Front Cover: Sample plug induced peak splitting in capillary electrophoresis studied using dual backscattered interferometry and fluorescence detection

Abstract

DOI: 10.1002/elps.202200244 The cover picture illustrates the use of two detection methods, laser induced fluorescence (LIF) and backscatter interferometry (BSI), combined to study mechanisms of peak splitting in capillary electrophoresis (CE). Simultaneous measurements from the same detection volume enable analyte peak splitting at the boundary of the sample plug and background electrolyte to be precisely measured. Systematic studies with dual detection CE are compared with a simple mathematical model to understand the origin of the peak splitting and develop strategies for its mitigation.