Abstract
Benzoic acid decarboxylases have been explored as efficient biocatalysts for the (de)carboxylation of electron-rich (hetero)aromatics. As members of the amidohydrolase superfamily, these enzymes share considerable structural and mechanistic features including the requirement of a divalent metal ion for catalysis. SEC-coupled ICPMS/MS measurements as well as crystal structure-based quantum chemical calculations revealed that two different metal ions (Mn2+ and Mg2+) can be embedded as catalytically active species in the active site of 2,3-dihydroxybenzoic acid decarboxylase from Aspergillus oryzae. The ability to utilise both Mn2+ and Mg2+ is illustrated by the two differently coloured half-spheres in the active centre of the enzyme. More information can be found in the communication by F. Himo, S. M. Glueck et al.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
