Front Cover: cyc‐DEP: Cyclic immunofluorescence profiling of particles collected using dielectrophoresis

Abstract

DOI: 10.1002/elps.202200001 The cover picture shows the collection of endogenous nanoparticles from blood using a dielectrophoretic chip that consists of a planar microelectrode array. Downstream immunolabeling reveals the diverse proteomic content of captured particles, represented by a spectrum of colors fluorescing at collection regions on the chip. Our group developed a technique called “cyc-DEP” to improve the multiplicity of on-chip proteomic analysis in what we believe is the first application of cyclic immunofluorescence for profiling liquid biopsies. Techniques like cyc-DEP showcase the potential utility of low-volume, minimally-invasive, lab-on-a-chip platforms for patient stratification in routine screening and early disease detection.