Abstract
Two immunologically distinct strains of E. maxima were examined in this study: the M6 strain and the Guelph strain. The differential expression between the sporozoites of the two strains of E. maxima was determined by image analysis of 100 μg of protein from each strain separated by standard one- and conventional two-dimensional polyacrylamide gel electrophoresis. In addition to differences in both molecular weight and the electrophoretic mobility, differences in the intensity of polypeptide bands for example, GS 136.4 and M6 169 were explored. Pooled gels were prepared from each strain. A representative 2D-PAGE gel spanning a non-linear pH range of 3–10 of E. maxima strain M6 consisted of approximately 694 polypeptide spots with about 67 (9.6%) of the polypeptide spots being unique relative to the other strain. E. maxima strain GS had about 696 discernable polypeptide spots with 69 spots (9.9%) that differed from those of the M6 strain. In-depth characterization of the variable polypeptide spots; unique polypeptide spots (absence or presence) and shared polypeptide spots with modifications may lead to novel vaccine target in the form of multi-component, multi-stage, multi-immunovariant strains, multi-species subunit vaccine, and diagnostic probe for E. maxima.
Highlights
Eimeria species are obligate intracellular apicomplexan protistan parasites
In this paper we explore the differences between two immunologically distinct strains of E. maxima; Guelph and M6 strains, by comparing the polypeptide spots of their sporozoites using one- and two-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D SDS-PAGE) to enhance our understanding of the previously demonstrated strain-specific nature of the immune responses of chickens to these strains
This study pertains to analysis of the protein profile of two immunologically distinct strains of E. maxima by 2D gel electrophoresis
Summary
Eimeria species are obligate intracellular apicomplexan protistan parasites. They are the major cause of chicken coccidiosis, a disease that leads to economic losses in livestock industries, poultry due to intensive rearing conditions. Weight loss, haemorrhage, general weakness, and death are some of the severe clinical signs of infection. The life cycle of PLOS ONE | DOI:10.1371/journal.pone.0143232. Exploring the Differences between Two Eimeria maxima Strains Using 2D The life cycle of PLOS ONE | DOI:10.1371/journal.pone.0143232 December 7, 2015
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