Abstract
On October 20, 1999, the Advisory Committee on Immunization Practices (ACIP) reviewed information about thimerosal in vaccines and received updates from CDC's National Immunization Program and several vaccine manufacturers on the current and anticipated availability of vaccines that do not contain thimerosal as a preservative. The review was prompted by a joint statement about thimerosal issued July 8, 1999, by the American Academy of Pediatrics (AAP) and the Public Health Service (PHS) (1) and a comparable statement released by the American Academy of Family Physicians (2). These statements followed a Congressionally mandated Food and Drug Administration (FDA) review of mercury in drugs and food, which included a reassessment of the use of thimerosal in vaccines.
Highlights
CARDIOVASCULAR DISEASE, INCLUDING coronary heart disease and stroke, is the leading cause of death in the United
Selection of laboratories was based on the type of method used for Hcy testing: high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and immunoassay
Three methods performed best regarding analytical precision: GC-MS, fluorescence polarization immunoassay (FPIA), and HPLC with fluorometric detection using a water-soluble phosphine as reducing agent (TCEP) and SBD-F as fluorescent tag
Summary
CARDIOVASCULAR DISEASE, INCLUDING coronary heart disease and stroke, is the leading cause of death in the United. Interlaboratory and intralaboratory (i.e., between tests run in a laboratory) variation was expressed as a relative standard deviation.* In the absence of target values for the samples analyzed, the GC-MS method was considered arbitrarily as a reference method. Because it used stable-isotopically labeled Hcy as an internal standard, this method is considered to be the most accurate and precise assay available. One HPLC method (HPLC with fluorescent detection using trialkylphosphine as a reducing agent and ABD-F as a labeling agent) produced results that were on average
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