From stored-product psocids to the other pests: the developments, problems and prospects on research and application of molecular identification:

Abstract

Psocids, beetles, moths and mites are regarded as the common kinds of stored-product pests in the world. The rapid and correct identification of stored-product pests is significant for quarantine, monitoring and control purposes. Molecular methods and techniques have been studied and applied for stored-product pest identification. Based on collection and analysis of literature in the last decade, this paper reviews the developments, questions and prospects for molecular identification of stored-product pests. As a representative model, the molecular methods and techniques for species identification of stored-product psocid pests were developed and applied systematically based on international collaboration involving China, Czech Republic, the United States and other countries. More than 10 studies on stored-product psocids related to RFLP, DNA barcoding, PCR, real-time PCR and gene chip have been published during this decade. Subsequently, DNA barcoding, PCR and real-time PCR techniques for the identification of common species of Tribolium and Cryptolestes pests of stored products have been reported by the same international team. Recently, a web system called Grain Pests DNA Barcode Identification System (GPDBIS) has been established in China using SOL SERVER and C#. Like a marathon that requires persistence, we should do our best to continue to promote research and application of molecular identification of stored-product pests with more international collaboration.