From precursor to peptide: Evidence for endomorphin biosynthesis?

Abstract

Event Abstract Back to Event From precursor to peptide: Evidence for endomorphin biosynthesis? Jayapalreddy Mallareddy1, Balazs Csibrany1, Attila Keresztes1 and Géza Tóth1* 1 Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Hungary Because of their high affinity, selectivity and agonist properties, endomorphins were proposed to be the endogenous ligands of the -opioid receptors. In spite of recent advances, the biosynthetic pathway of endomorphins is still unclear. Recent findings suggested that endomorphin-2 may be produced from a human sulfate transporter protein in consecutive enzymatic reactions. Many biologically active neuropeptides and peptide hormones were shown to be synthesized from inactive glycine-extended precursors, which require a carboxy-terminal posttranslational amidation for biological activity. This reaction is catalyzed by a consecutive action of peptidylglycine-α-hydroxylating monooxygenase (PHM) and peptidylamidoglycolate lyase (PAL). These two enzymes are part of the peptidylglycine α-amidating monooxygenase (PAM), a bifunctional, integral membrane-bound enzyme machinery. In attempt to elicit the hypothesis that endomorphin-2 may be produced from the sulfate transporter precursor protein by the activation of the PAM enzyme system we designed and performed a series of biochemical experiments. Biological matrices, serum and brain homogenates were prepared for the in vitro metabolic studies. Endomorphin-2, Gly5-extended endomorphin-2 as well as their Dmt1-containing analogs were synthesized using SPPS methods. The biodegradation of the Gly5-extended endomorphin-2 was monitored by RP-HPLC and MALDI-TOF mass spectroscopy. Additionally, the -and -opioid receptor binding affinities of the aforementioned analogs were tested. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Mallareddy J, Csibrany B, Keresztes A and Tóth G (2009). From precursor to peptide: Evidence for endomorphin biosynthesis?. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.245 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2009; Published Online: 13 Mar 2009. * Correspondence: Géza Tóth, Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary, geza@brc.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jayapalreddy Mallareddy Balazs Csibrany Attila Keresztes Géza Tóth Google Jayapalreddy Mallareddy Balazs Csibrany Attila Keresztes Géza Tóth Google Scholar Jayapalreddy Mallareddy Balazs Csibrany Attila Keresztes Géza Tóth PubMed Jayapalreddy Mallareddy Balazs Csibrany Attila Keresztes Géza Tóth Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.