Abstract
s of Pharma Nutrition 2013 / PharmaNutrition 2 (2014) 75–119 81 functions. Using the human epithelial intestinal cell line Caco-2 in a transwell system as an in vitromodel, we studied the effect of DON in the presence and absence of galacto-oligosaccharides (scGOS). Parameters assessed included transepithelial electrical resistance (TEER), paracellular tracer flux, expression of tight junction proteins, cytokine release and overall cell viability. Interestingly, when a DON challenged non-permeable Caco-2 monolayer was preincubated with scGOS for 24h, TEER and paracellular transport of the marker lucifer yellow remained unaffected. In parallel, the levels of claudin-3 mRNA expression were not affected by DON exposure in the presence of scGOS. Furthermore, the increase in epithelial IL-8 mRNA expression and in IL-8 secretion, observed after DON exposure, was counteracted by scGOS. In conclusion, we demonstrated with this in vitro model that scGOS protects and restores the epithelial barrier dysfunction exerted by the food contaminant DON. In addition, the hypothesis that scGOS has antiinflammatory properties was confirmed by demonstrating that the DON-induced production of IL-8was counteracted by scGOS. Taken together, these findings indicate that dietary oligosaccharides may be a promising approach to mitigate the effects of undesirable dietary mycotoxin exposure.
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