From Molecules to Mating Success: Integrative Biology of Muscle Maturation in a Dragonfly

Abstract

2 SYNOPSIS. Dragonflies begin adult life as comparatively weak fliers, then mature to become one of nature's ultimate flying machines. This ontogenetic transition provides an opportunity to investigate the relationship between life history, phenotypic plastic- ity, and changing ecological demands on organismal performance. Here we present an overview of a wide-ranging study of dragonfly muscle maturation that reveals i) ecological changes in the need for efficient versus high-performance flight, ii) organ- ism-level changes in performance, thermal physiology, locomotor mechanics, and en- ergy efficiency, iii) tissue-level changes in muscle ultrastructure and sensitivity to ac- tivation by calcium, and iv) molecular-level changes in the Lsoform composition of a calcium regulatory protein in flight muscle (troponin-T). We discuss how these phe- nomena may be causally related, and thereby begin to show linkages across many levels of biological organization. In particular, we suggest that alternative splicing of troponin-T mRNA is an important component of the of muscle contractile function for developmental changes in wingbeat frequency and ecological demands on flight performance. Age-variable gearing of muscle function allows energetically eco- nomical flight during early adult growth, whereas power output is maximized at ma- turity when aerial competition determines success during territoriality and mating.