Abstract
Feather is a waste product generated in large quantities from industrial poultry processing. Recycling of this renewable source of biopolymers has been the objective of many researches due to its high protein content, biodegradability, and biocompatibility. This study investigates the feasibility of producing nanoparticles from feather waste by enzymatic hydrolysis, followed by ultrasonic treatment. The effects of enzyme concentration, hydrolysis time and substrate concentration on particles size were evaluated to optimize the best condition in order to attain the smallest particles by a Box-Behnken Design. The optimum hydrolysis conditions were found to be: enzyme concentration: 3.6%, substrate concentration: 5 g/l and hydrolysis time: 243 h. Scanning electron micrographs indicated fiber fibrillation and degradation as it was progressively converted into particles form. The results of particle size analysis indicated the positive effect of sonication on reducing particles size. Fourier transform infrared spectra showed no remarkable changes in the chemical composition of treated samples. Moreover, crystallinity and thermal stability of feather nanoparticles enhanced upon enzymatic hydrolysis and ultrasonic treatment.
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