From Farm to Fork: Enhancing Agricultural and Food Safety Monitoring with Laser-Engineered Graphene Sensors

Abstract

In the critical fields of precision agriculture and food safety, the demand for accurate and sensitive monitoring tools for fertilizers, pesticides, and food-borne pathogens cannot be overstated. Our collaborative research with commercial entities seeks to overcome the challenges faced by current electrochemical ion-selective, enzymatic, and immunosensors, which often fall short in selectivity, stability, and sensitivity, particularly under field conditions. We showcase the tunable properties of Laser-Induced Graphene (LIG) – such as surface wettability, roughness/area, and electrical conductivity – and their instrumental role in enhancing the commercial viability of these sensor technologies.We demonstrate the conversion of hydrophilic Laser-Induced Graphene (LIG) to a near-superhydrophobic state using an innovative dual-laser process in ambient conditions. This method intricately sculpts the surface into micro and nanoscale structures reminiscent of a lotus leaf, resulting in nearly superhydrophobic characteristics. By modifying the laser's power, speed, and fluence, we can also fine-tune the electroactive surface area and electrical conductivity, significantly impacting sensor functionality. For solid contact ion-selective electrodes (SC-ISEs), enhancing the LIG surface with high hydrophobicity prevents water layer accumulation at the ion-selective membrane and electrode interface. This design expels water from within the membrane, fosters stronger membrane-electrode adhesion, and consequently, diminishes signal interference. Such improvements are instrumental in boosting the sensor's accuracy and extending its operational lifespan. In contrast, a hydrophilic LIG with a large surface area enhances catalytic efficiency and allows more biorecognition agents to adhere to its surface, thereby amplifying the sensitivity of enzymatic pesticide sensors. Lastly, a flat, hydrophilic LIG surface ensures an even distribution of antibodies, which enhances the performance of sensors detecting foodborne pathogens.These modifications to LIG and the resulting sensors have substantial commercial potential. We have developed ions sensors for nitrate, ammonium, potassium, calcium, and magnesium have demonstrated near-Nernstian sensitivities, extensive sensing ranges across four orders of magnitude, and the ability to detect concentrations as low as 1 ppm in soil and water samples. These sensors are currently being integrated into commercial soil moisture and temperature probes for monitoring in farm fields. The pesticide sensing capability of the sensor can reach an extremely low picomolar detect limit for organophosphates, the nanomolar regime for neonicotinoids, and micromolar regime for glyphosate in surface waters. These sensors are currently being interfaced with LIG-based mist/fog harvesting devices to monitor pesticide and spray drift in farm fields. Finally, the LIG-based Salmonella sensors are capable of monitoring bacteria such as Salmonella to about 10 CFU/mL in actual food matrices such as chicken broth without the need for sample preconcentration or costly lab analyses.This research forges innovative avenues in agriculture and food safety monitoring, laying the groundwork for mass production of efficient and economical sensors. We believe this technology will revolutionize field monitoring, significantly advancing sustainable agriculture and improving public health safety. Figure 1