Abstract
(1) Background: To evaluate the effects of the direct and indirect contact of leukocyte and platelet-rich fibrin (L-PRF) on bone development, in an ex vivo embryonic chick femur model. (2) Methods: Both sections of L-PRF membranes (red and yellow portions) were evaluated with scanning electron microscopy and histochemical staining. The in vivo angiogenic activity was evaluated using a chorioallantoic membrane model. The osteogenic activity was assessed with an organotypic culture of embryonic chick femora through direct and indirect contact, and assessment was conducted by microtomographic and histological analysis. Descriptive statistics, One-Way ANOVA and Tukey’s multiple comparisons tests were performed for datasets that presented a normal distribution, and Kruskal-Wallis tests were performed for non-parametric datasets. A significance level of 0.05 was considered. (3) Results: The L-PRF induced angiogenesis reflected by a higher number and a larger and more complex gauge in the vessels that invaded the membrane. The physical presence of the membrane over the bone (direct contact) unleashes the full potential of the L-PRF effects on bone growth enhancement. The greatest increase in mineral content was observed in the diaphysis region. (4) Conclusion: The L-PRF direct contact group presented higher values on mineral content for bone volume, bone surface and bone mineral density than the indirect contact and control groups.
Highlights
Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, LAQV/REQUIMTE, University of Porto, 4160-007 Porto, Portugal
leukocyte and platelet-rich fibrin (L-platelet rich fibrin (PRF)) membranes were characterized into red and yellow portions based on their morphological aspects
The red portion (Figure 1A-Red) presented several blue-stained nuclear leukocytes, distributed across the upper region of the L-PRF, which were rarely identified in the yellow portion (Figure 1A-Yellow)
Summary
This study aims to assess the L-PRF modulation capability, either directly or indirectly, on the bone development of the embryonic chick femur, grown ex vivo. This study aimed to characterize the L-PRF membrane and investigate its angiogenic. This study aimed to characterize the L-PRF membrane and investigate its angiogenic potential using an in vivo CAM model, as well as its osteogenic activity on the chick empotential using an in vivo CAM model, as well as its osteogenic activity on the chick bryo femur model, assigning this ex-vivo system as a viable alternative method to further embryo femur model, assigning this ex-vivo system as a viable alternative method to studies on L-PRF-mediated bone development and regeneration
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