Abstract
Surface plasmon resonance (SPR) for biosensing was demonstrated 30 years ago. In the present contribution, its general background is described together with the necessary developments both in instrumentation and surface chemistry, leading to the final so-called BIAcore technology. The description is naturally colored by my personal opinion of the developments. SPR for the elucidation of organic mono- and multilayers introduced at the end of the 1970s formed the basis for the first biosensing demonstration of SPR in the beginning of the 1980s. It is pointed out how the need of an up-to-date laboratory exercise for the undergraduate students and the multidisciplinary environment at the Laboratory of Applied Physics at Linköping University led to this demonstration. The initial experiments are touched upon and the further developments at Pharmacia, which led to the BIAcore technology, are described in some details. Some of the present activities in Linköping related to optical biosensing with ubiquitous instrumentation are also described, including SPR detection with a computer screen and a web camera and most recently with a cellular phone.
Highlights
Surface plasmon resonance (SPR) for biosensing was demonstrated 30 years ago
& Developments in making surface plasmon resonance into a pioneering biosensor technology & Later activities in Linköping & Conclusions. It should be pointed out already in the “Introduction” that the original demonstration was made in the multidisciplinary environment at the Laboratory of Applied Physics, it was the contribution from Pharmacia which made SPR into a pioneering biosensor technology
Some of the first applications of ellipsometry in the new laboratory were related to protein adsorption as a function of surface energy, model studies of surface-induced blood coagulation, and complement activation related to biomaterial surfaces [9,10,11]
Summary
The encouraging gas sensing results, discussions with and suggestions from microbiologists at the Laboratory of Applied Physics, led us to try immune sensing with SPR, which resulted in a “classical” paper [1]. The initial experiments were made with a silver film evaporated on a microscope slide, which was used as one wall of a cuvette through which water solutions could be flown. The resonance angle shift upon adsorption of IgG was 0.6°, which suggests an organic layer thickness of about 50 Å, a reasonable value for a monolayer of IgG. The binding of a-IgG caused a further shift of almost 1°. The analytical capabilities of the setup were elucidated using the initial kinetics of the resonance angle shift upon addition of the a-IgG. The angle of incidence was kept constant at the left part of the resonance curve with adsorbed IgG and the increase in light intensity upon addition of a-IgG measured for different a-IgG concentrations. It was concluded that “we have demonstrated selective antibody reactions with a sensitivity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
