Abstract
AbstractOur overall objective is to investigate whether modulation of neuronal activity in the superior colliculus (SC), the principal retinal target structure and main extraretinal neurotrophic factor source in mice, can protect against chronic RGC loss, experimentally induced using the well‐establised optic nerve crush (ONC) mouse model of glaucoma. For activity modulation, we will make use of the novel revolutionary technology of optogenetics. More precisely, in this project we aim to: 1) validate the genetic labeling of SC neurons with AAV vectors, carrying an stable‐step function opsin (SSFO) under control of a neuronal (CMV) promoter; 2) test the ability to chronically stimulate the transduced cells; and 3) evaluate the potential of chronic optogenetic modulation of SC neuronal activity to promote RGC survival in the optic nerve crush model.
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