Abstract
Inhibitors of cAMPand cGMP-phosphodiesterases (PDEs) are important substances which are in clinical use or under development for the therapy of plethora of diseases, such as erectile dysfunction, heart failure and memory disorders. However, there are very few methods available to screen new substances for their PDE inhibitory activity in intact living cells. Current methods using reporter cell lines are rather indirect and based e.g. on calcium imaging of cyclic nucleotide gated channel activity [1].
Highlights
3rd International Conference on cGMP Generators, Effectors and Therapeutic Implications Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here.
Inhibitors of cAMP- and cGMP-phosphodiesterases (PDEs) are important substances which are in clinical use or under development for the therapy of plethora of diseases, such as erectile dysfunction, heart failure and memory disorders
To develop a new strategy for PDE-inhibitor screening, we used fluorescence resonance energy transfer (FRET)-based sensors for cAMP and cGMP, which consist of a single cAMP/cGMP-binding domain flanked by a pair of green fluorescent protein mutants [2,3]
Summary
3rd International Conference on cGMP Generators, Effectors and Therapeutic Implications Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. . Address: Department of Pharmacology, University of Würzburg, Würzburg, D-97078, Germany Email: Viacheslav O Nikolaev* - nikolaev@toxi.uni-wuerzburg.de * Corresponding author from 3rd International Conference on cGMP Generators, Effectors and Therapeutic Implications Dresden, Germany.
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