Abstract
Abstrac 1. 1. In the gill of the euryhaline teleost, Chelon labrosus, the basic characteristics of acid phosphatase activity, a “lysosomal” enzyme, are similar to those of the other vertebrates. 2. 2. The pH optimum is 4.75; the enzyme activity reaches a plateau from pH 3.8 to pH 5.0; at higher pH levels, enzyme activity rapidly declines. 3. 3. Normal kinetic patterns of enzyme activity occur when substrate, enzyme concentration and time of incubation in enzyme assays are varied. The ions (Na + K +, Cl −, Ca 2+ and Mg 2+) and chelators (EGTA, EDTA). do not affect the enzyme activity. Decrease (12% to 25%) in in vitro enzyme activity occurs after one day at 0°-4°C. 4. 4. Acid phosphatase activity, found above all 50,000 g × 1 hr fraction and moved in slighter subcellular fraction after in vitro osmotic shock, is probably localyzed within the lysosomes. 5. 5. The specific activity increases when fish is transferred from sea water to freshwater.
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