Abstract
Abstract The study was conducted to determine the quality characteristics of fresh and post-thaw semen of Holstein Friesian bull, maintained at Semen Production Unit, Quetta Baluchistan, Pakistan. The semen were evaluated for volume, pH, mass activity, sperm concentration, sperm motility and integrity of sperm cell membrane. The semen qualifying these criteria were processed for freezing. The frozen semen after thawing was assessed for progressive linear motility and intact cell membrane. The mean ejaculate volume of semen was recorded as 6.93, 6.59 and 6.61 ml, pH; 6.58, 6.59 and 6.61, mass activity of sperms 3.87, 3.75 and 3.5 and sperm concentration 1349.12x106, 1509.37x106 and 1364.37x106 /ml during the month of May, June and July respectively. The color of semen was not consistent pattern, it varied as creamy white and milky white. The motility of fresh and post thaw semen was assessed as 73.9 vs 48.75%; 75 vs. 53.75%; 75 vs. 53.12% for the month of May, June and July respectively. The membrane integrity of fresh semen samples over a period of three month were 58.37% against post-thaw membrane integrity of 44.79%. It was concluded that the semen of Holstein Friesian bull tolerates freezing and thawing stress and maintained reasonable good fertility. The summer season has no significant adverse effect on quality of semen. Keywords: Bull; Fresh; Holstein Friesian; Post-thaw; Quality; Semen http://dx.doi.org/10.19045/bspab.2019.80020
Highlights
1349.12x106, 1509.37x106 and 1364.37x106 /ml during the month of May, June and July respectively
The bulls were maintained at semen production unit Quetta, Baluchistan
Fresh and post thaw quality of Holstein Friesian bull semen were evaluated for volume, pH, mass activity, sperm concentration, sperm motility and membrane integrity
Summary
1349.12x106, 1509.37x106 and 1364.37x106 /ml during the month of May, June and July respectively. The motility of fresh and post thaw semen was assessed as 73.9 vs 48.75%; 75 vs 53.75%; 75 vs 53.12% for the month of May, June and July respectively. The number of bulls required for breeding improve the techniques of insemination and purpose, greatly less and the quality of diluted semen [2, 3]. Semen evaluation Immediately after collection the semen samples were transferred in to the water bath, kept at 37 0C and evaluated for volume, color, pH, sperm concentration, mass activity, sperm motility and progressive motility percentage of spermatozoa. Assessment of fresh semen samples Appearance Each fresh semen sample was assessed with naked eyes Any unusual things such as blood, dis-coloration, dirt, dust and debris material was noted. Volume Semen volume was measured directly from graduated collecting tube and recorded in ml
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