Abstract
BackgroundY-chromosome STRs are valuable in the forensic identification of male DNA from sexual assault cases, and they are used to link families through genetic genealogy.Materials and methodsFor Y-STR analysis, 1032 male blood samples were used in this study, direct PCR technique was used for DNA amplification using the PowerPlex® Y23 System, and then PCR product was run with Genetic Analyzer, and the data were analyzed with the Gene Mapper ID Analysis Software. Frequency-based statistical analysis was calculated with GenAlEx 6.5-Genetic Analysis.ResultsOne hundred and eighty-five alleles were detected at the 23 Y-STR loci in 1032 samples. Alleles frequency ranged from 0.002 to 0.813 and the highest allele frequency registered (0.813) for allele 11 at locus DYS392, and the mean haplotype diversity was 0.616 ± 0.027. New variants were registered for DYS458 locus.ConclusionThe present study established the genetic information obtained by using the PowerPlex® Y23 System for the Iraqi population and also created a database of 23 Y STR markers in this population.
Highlights
Y-chromosome Short tandem repeats (STRs) are valuable in the forensic identification of male DNA from sexual assault cases, and they are used to link families through genetic genealogy
Y-STR markers are located on the non-recombining region (NRY) of the Y chromosome; they are transmitted as haplotypes in the same way as single locus alleles, and the firm paternal inheritance of Y-STRs indicates their importance in paternity and kinship tests (Gusmão et al, 2006; Jain et al, 2016)
Four different profiles were detected each within 6 individuals, which could be considered as accepted result due to the high inbreeding rate between family members or clan members across the country
Summary
Y-chromosome STRs are valuable in the forensic identification of male DNA from sexual assault cases, and they are used to link families through genetic genealogy. Requests for kinship testing between different family members are sent from courts of law and police stations from all over Iraq to Paternity and Kinship division in Medico-Legal Directorate (MLD), Baghdad, to establish the identity of an unregistered people or to solve a familial dispute. In many of these cases, the use of DNA short tandem repeat (STR) is not enough to resolve complicated cases. We represent the frequency of alleles registered in each locus in Iraqi male samples that were collected from all over the country (except Kurdistan), which may provide more information about the genetic pool of the Iraqi population
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
