Abstract
Klaten Regency is an area in Central Java Province with a high rate of endemic dengue hemorrhagic fever. This study aims to demonstrate a correlation between transovarial transmission and the incidence of dengue haemorrhagic fever in Ceper District, Klaten Regency. Aedes larvae and pupae were collected from January 2019 to March 2019 from various containers, using gamadotic and dipper tools, in Ngawonggo, Kajen, and Meger villages, which have high, medium, and low numbers of dengue cases, respectively. The larvae and pupae obtained were reared into adults. The presence of dengue virus in head squashes of 1-week-old female Ae. aegypti was identified based on an immunocytochemical assay using DSSE10 monoclonal antibody, made at Universitas Gadjah Mada as a primary antibody. The frequency of transovarial transmission of dengue virus (TIR) in Ae. aegypti in the three villages was 35.7%. The highest frequency occurred in Ngawonggo Village, at 40 %, and the lowest in Meger Village, at 28%, while the frequency in Kajen Village was 36.9 %. This study proves that there is a strong correlation (r = 0.99664) between transovarial transmission and the incidence of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java.
Highlights
Dengue haemorrhagic fever (DHF) is an important health problem in tropical and subtropical countries, including Indonesia
This condition may be related to the role of transovarial transmission of dengue virus (DENV) in the dengue vector during the epidemic of DHF
Based on the preceding explanation, the objective of this study is to apply the immunocytochemical technique for virological surveillance through detection of transovarial infection in the endemic dengue area of DHF in Kecamatan Ceper, Klaten Regency, and to apply a geographical information system to describe the influence of high transovarial transmission on the incidence of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java
Summary
Dengue haemorrhagic fever (DHF) is an important health problem in tropical and subtropical countries, including Indonesia. According to the Central Java Health Office (2016), Klaten Regency in Central Java Province is an area with high endemic DHF [2]. Klaten Health Office increased the source reduction of mosquito breeding sites and formed Juru pemantau jentik (larval surveyor) in 2017, and this activity succeeded in reducing dengue cases to 101 cases with three deaths [3]. This condition may be related to the role of transovarial transmission of dengue virus (DENV) in the dengue vector during the epidemic of DHF. There have been few studies involving transovarial transmission and viral vector monitoring as a surveillance tool and control strategy
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