Frequency of occurrence of the PPAR family gene polymorphisms and their relationship with lipid levels in healthy young individuals

Abstract

Objective . To determine the frequency of occurrence of the genotypes of the PPARG (Pro12Ala), PPARD (294T/C), and PPARA (G2528C) gene polymorphic variants, and to establish their relationship with blood lipid levels in healthy young individuals. Materials and methods . The study included healthy young individuals (n=90) aged 20.1 ± 0.6 years. The parameters of the lipidogram were evaluated: total cholesterol (TC), low-density lipoprotein cholesterol (LDL cholesterol), highdensity lipoprotein cholesterol (HDL cholesterol), triglycerides (TGs), atherogenicity coefficient (CA). The polymorphic variants of the PPAR genes were determined. The results of the study were processed using the STATISTICA 10.0 application package. Results . The genotype distribution of the Pro12Ala polymorphic variant of the PPARG gene (χ 2 =0.0079; p=0.73), 294T/C of the PPARD gene (χ 2 =0.26; p=0.65), and G2528C of the PPARA gene (χ 2 =4.72; p=0.8) in the healthy young individuals corresponded to the expected Hardy-Weinberg equilibrium. No significant differences between the lipid levels in carriers of different variants of the polymorphic PPARG gene (Pro12A1a) were obtained. Conclusion . Individuals with the C/C polymorphic variant had significantly higher TG levels, lower HDL cholesterol levels in the blood, and the highest CA compared with carriers of the T/T and T/C variants of the PPARD (294T/C) polymorphic gene. Carriers of the C/C polymorphic variant had significantly (p=0.049) higher LDL-C levels than carriers of the G/C variant of the PPARA (G2528C) polymorphic gene. The risk of hypertriglyceridemia is 9.6 times, of low HDL-C is 6.9 times, and of having an elevated CA rate is 5.2 times as high in individuals with the C/C polymorphic variant as in individuals with the T variants /T and T/C of the PPARD gene (294T/C).