Frequency of NOD2/CARD15 gene polymorphisms in the Algerian pediatric Crohn's disease patients

Abstract

Event Abstract Back to Event Frequency of NOD2/CARD15 gene polymorphisms in the Algerian pediatric Crohn's disease patients Amira Bouzidi1* 1 USTHB, Algeria Introduction Population-based studies have provided compelling evidence that genetic factors contribute to the pathogenesis of Crohn’s disease (CD). NOD2/CARD15 an intracellular receptor that contributes to host defense was identified as the first susceptibility gene for CD. The aim of this study is to describe for the first time, the allele frequency of most relevant SNPs in NOD2 gene in the Algerian Paediatric CD population and healthy people. Material and methods 20 unrelated pediatric CD patients with a mean age of 12.5 (±4.2) years and 20 healthy controls were enrolled in this study after obtaining informed consent. NOD2 polymorphisms (C2104T, G2722C, 3020insC, C802T) were analyzed using PCR-RFLP method. Results The 2104T allele is detected in both CD and healthy population but is more frequent in CD patients than controls (10% vs 5%). Neither G2722C nor 3020insC mutations are found in patients but they are detected in two different healthy controls. The 802T allele is found in both population but with a slightly higher frequency in the control group (30% vs 37%). Conclusion Our study shows that allelic variants of NOD2 are present in the Algerian population and their frequency varies between CD patients and controls. However, NOD2/CARD15 doesn’t seem to be a genetic susceptibility factor in the Algerian pediatric CD patients. We suggest that further genetic characterizations maybe needed for Algerian CD patients. Keywords: pediatric Crohn's disease, NOD2/CARD15, Polymorphism, Single Nucleotide, PCR-RFLP, IBD Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Poster Presentation Topic: Pediatric immunology Citation: Bouzidi A (2014). Frequency of NOD2/CARD15 gene polymorphisms in the Algerian pediatric Crohn's disease patients. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00014 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Miss. Amira Bouzidi, USTHB, Algiers, Algeria, amirabzd@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Amira Bouzidi Google Amira Bouzidi Google Scholar Amira Bouzidi PubMed Amira Bouzidi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.