Frequency of induced mutations at the haploid and diploid levels inNicotiana tabacum L.

Abstract

The frequencies of chlorophyll mutants were investigated in anther cultures derived from mutagen-treated plants ofN. tabacum cv. Samsun (haploid level) and in the seed offspring from the same treated plants (diploid level). Comparison of the induced mutation frequencies at the haploid and diploid levels demonstrated that selection existed against the haploid embryoids with induced chlorophyll deficient mutations. The diploid vegetative stage with phenotypic expression of the chlorophyll mutation was more vital than the haploid one. The suitability of anther cultures for studying induced mutagenesis is discussed.