Abstract
Background Squamous cell carcinoma of the oral cavity and oropharynx is a public health issue, with over 200,000 new cases worldwide each year [1]. Studies show that the incidence is higher in male individuals, aged between 40 and 55 years. The consumption of tobacco and alcohol are wellestablished risk factors for developing head and neck cancer, and studies have been shown synergism between these substances, which contributes to the increased risk [4]. Recently, persistent infection with high risk Human Papillomavirus (HPV) has been identified as a potential risk factor for the development of these tumors [2]. HPV is a DNA virus that features tropism to epithelial cells. The high risk HPV types, such as types 16 and 18 have the ability to integrate their DNA into the host cell DNA, immortalizing keratinocytes, with great possibilities of causing a cancer by infecting cells [3]. This study aims to evaluate the frequency of HPV infection, smoking and alcohol consumption related to the development of squamous cell carcinomas of the oral cavity and oropharynx.
Highlights
Squamous cell carcinoma of the oral cavity and oropharynx is a public health issue, with over 200,000 new cases worldwide each year [1]
Electrophoresis was performed in 1% agarose gel containing ethidium bromide, to confirm the amplification and quality of extracted DNA, the DNA samples were subjected to nested PCR with primers MY09/11 and GP5 + / GP6 + for viral DNA detection of Human Papillomavirus (HPV) subtypes 16 and 18
Samples known to be positive for both subtypes of HPV 16 and 18 were used positive controls
Summary
Squamous cell carcinoma of the oral cavity and oropharynx is a public health issue, with over 200,000 new cases worldwide each year [1]. The consumption of tobacco and alcohol are wellestablished risk factors for developing head and neck cancer, and studies have been shown synergism between these substances, which contributes to the increased risk [4]. Persistent infection with high risk Human Papillomavirus (HPV) has been identified as a potential risk factor for the development of these tumors [2]. The high risk HPV types, such as types 16 and 18 have the ability to integrate their DNA into the host cell DNA, immortalizing keratinocytes, with great possibilities of causing a cancer by infecting cells [3]. This study aims to evaluate the frequency of HPV infection, smoking and alcohol consumption related to the development of squamous cell carcinomas of the oral cavity and oropharynx
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