Abstract
Introduction: The purpose of this research was to analyze blood-group antigen-binding adhesion (babA2) and sialic acid binding adhesion (sabA) genotypes status in Helicobacter pylori (H. pylori) isolates and their relationship with clinical outcomes. Methods: Gastric biopsy specimens were homogenized and placed in Brucella agar medium supplemented with 5% sheep blood and 3 antibiotics and were cultured at 37 °C under microaerophilic conditions and incubated for 4-7 days. H. pylori was identified by typical morphology, gram-staining and urease tests, and babA2 and sabA genes were detected by polymerase chain reaction (PCR). Results: From a total of 100 H. pylori isolates; babA2 and sabA genes were detected in 23.0 and 26.4%, respectively. There was a significant relationship between these genes and clinical outcomes (P < 0.050). Conclusion: We found that the babA2 status was not related to clinical outcomes in Tabriz, Iran. However, sabA was a promoting determinant for disease, and multivariate analysis disclosed sabA to be an independent marker of non-ulcer diseases in our subjects.
Highlights
The purpose of this research was to analyze blood-group antigen-binding adhesion and sialic acid binding adhesion genotypes status in Helicobacter pylori (H. pylori) isolates and their relationship with clinical outcomes
Gastric biopsy specimens were homogenized and placed in Brucella agar medium supplemented with 5% sheep blood and 3 antibiotics and were cultured at 37 °C under microaerophilic conditions and incubated for 4-7 days
H. pylori was identified by typical morphology, gram-staining and urease tests, and babA2 and sialic acid binding adhesion (sabA) genes were detected by polymerase chain reaction (PCR)
Summary
The purpose of this research was to analyze blood-group antigen-binding adhesion (babA2) and sialic acid binding adhesion (sabA) genotypes status in Helicobacter pylori (H. pylori) isolates and their relationship with clinical outcomes. Gastric biopsy specimens were homogenized and placed in Brucella agar medium supplemented with 5% sheep blood and 3 antibiotics and were cultured at 37 °C under microaerophilic conditions and incubated for 4-7 days. H. pylori was identified by typical morphology, gram-staining and urease tests, and babA2 and sabA genes were detected by polymerase chain reaction (PCR).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
