Abstract
This study evaluated the frequency of intestinal parasites, emphasizing the identification and differentiation of Entamoeba spp. Multiplex polymerase chain reaction (PCR), coproantigen tests and morphometric analysis were performed for Entamoeba spp. differentiation. The overall frequency of intestinal parasites was 65%. Entamoeba histolytica was detected by the coproantigen test, and the PCR showed that Entamoeba dispar predominated in the population. In contrast, morphometric analysis was important for identifying Entamoeba hartmanni. It is possible to identify the causative agent of amoebiasis and to differentiate this agent from other species by combining techniques.
Highlights
This study evaluated the frequency of intestinal parasites, emphasizing the identification and differentiation of Entamoeba spp
All samples that tested positive for the E. histolytica/E. dispar complex were stained with iron hematoxylin
A morphometric analysis was performed on slides prepared from the twenty-four samples that tested positive for the E. histolytica/E. dispar complex
Summary
This study evaluated the frequency of intestinal parasites, emphasizing the identification and differentiation of Entamoeba spp. Entamoeba hartmanni has smaller dimensions and can be differentiated from the E. histolytica/E. dispar complex through the staining and morphometric analysis of cysts and trophozoites. All samples that tested positive for the E. histolytica/E. dispar complex were stained with iron hematoxylin.
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