Abstract
Objective: To determine the frequency of Acid Fast Bacilli (AFB) in samplessubmitted for Ziehl Neelson (Z.N) Technique. Design: An Observational study. Place andduration of study: This study was carried out from july 2010 to Dec 2012 at Dept. of Clinicalpathology Main Laboratory Jinnah Post Graduate Medical Centre (JPMC) Karachi. Material &Method: A total of 5064 samples were received in the department of clinical pathology mainlaboratory, JPMC, Karachi during the study period. We determined the presence of AFB insamples by Z.N Technique. Result: Out of total 5064 samples, 518(10.2%) specimens revealedthe presence of AFB. The samples received were: sputum 4787 (503 positive; 10.5%), pus 56 (01positive; 1.7%), fluid 118 (no positive), gastric 93 (14 positive; 15%); urine 10 (not positive).Conclusions:- Lower rate of positivity for Acid Fast Bacilli by Z.N Technique can be increased byFluorescence microscopy and culture technique.
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