Frequency evaluation of the CYP3A4*4 polymorphism in iranian healthy volunteers

Abstract

Purpose: CYP3A4 (cytochrome P450, family 3, subfamily A, polypeptide 4) is an important enzyme in the body. The purpose of this enzyme is to oxidize small foreign organic molecules such as drugs or toxins. The different genetic variants CYP3A4 present in individuals such as CYP3A4*4. The cytochrome P450 3A subfamily have an important role in the catabolic reactions like many of peroxidative, oxadative, and reductive biotransformation reactions of common drugs such as Carbamazepine, Hydroxylations and Nevirapine. Methods: In this study, the prevalence of CYP3A4*4 in healthy subject of Iran were analyzed. Three hundred healthy unrelated subjects were chosen. After DNA extraction, genotypes were analyzed by PCR-RFL Pand PCR-sequencing. Results: No mutation was detected for CYP3A4*4 (Ile118Val) in these individuals. This study can be a background for future studies specially pharmacogenomic investigations and association studies. In addition, this data could be help clinicians optimize therapy or recognition persons who have risk of adverse drug reactions. Conclusions: Our results show that the frequencies of the CYP3A4*4 polymorphism in Iranian population were almost similar to the other populations such as Malaysian, Indian, Taiwanese, Tepehuan Amerindians and Mestizo Mexicans. CYP3A4*4 mutation causes decrease enzyme activity in vivo because the Ile118Val mutation may affect the substrate binding and cause decrease in CYP3A4 activity. Therefore, lack of the CYP3A4*4 mutation among Iranian population renders the consumption of drugs whose metabolismis done by CYP3A4, harmless.