Abstract
The effects of alternating magnetic field (AMF) stimulation on the proliferation of human liver cancer cells (HepG2) under varying frequencies from 500 ㎐ to 2 ㎑ were investigated. The AMF stimulator generated a magnetic field of 5 mT with a saw tooth pulse waveform, the frequency of alternating pulses was controlled by the duty factor of the digital control circuit. HepG2 cells were cultured in a 6-well plate over a magnetic coil using a cooling system while been kept for 48 hours in an incubator. After magnetic stimulation of 0.5, 1 and 2 ㎑, the proliferation rates of the HepG2 cells saw almost no difference compared to liver cancer cells that were not stimulated. However, the proliferation rates at 1.4 ㎑ were reduced by up to 66 % compared to the non-stimulated cells. In other words, HepG2 cancer cells can be suppressed by magnetic stimulation of a certain frequency. These results are in good agreement with those from analysis of cell viability using MTT assay, a colorimetric assay used for assessing cell metabolic activity. This phenomenon of a suppression frequency may be related with various ionic flows that occur in the ion channels of cell membranes.
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